Genotypic and Phenotypic Characterization of Mammaliicoccus sciuri - A MDR Strain causing Clinical Mastitis in Cows of Odisha, India

Background: Mammaliicoccus sciuri is a multidrug resistant human pathogen. Literature on its role causing bovine mastitis scarce. Detection of M. from cow with clinical in Malkangiri district Odisha stimulated to ascertain status larger population and study characteristics genotypic phenotypic levels. Methods: 520 lactating cows various herds Odisha, India were screened for presence mastitis. Milk samples collected aseptically 15 16S rRNA amplification was performed know bacterial pathogen (s). Conventional PCR carried out detect resistance genes against β-lactam (blaZ), aminoglycoside (aacA-aphD) tetracycline (tetK, tetM). In vitro sensitivity test studied isolates using nine antibiotic discs. Phylogenetic analysis compare origin. Result: isolated 6 (40.0%) positive milk samples. showed highest degree penicillin (100%) followed by, cefoperazone (33.3%), streptomycin (16.0%). One isolate had tetK, tetM gene. (75-100%) similarity other obtained GeneBank. Present highlights tetK gene sciuri.